StressCovidV1, Main, Exploration, bibRecord, 003292

Role of hydrophobic interactions in the fusion activity of influenza and Sendai viruses towards model membranes.

Identifieur interne : 003292 ( Main/Exploration ); précédent : 003291; suivant : 003293

Role of hydrophobic interactions in the fusion activity of influenza and Sendai viruses towards model membranes.

Auteurs : J. Ramalho-Santos [Portugal] ; R. Negrão ; M. Da Conceição ; P. De Lima

Source :

Bioscience reports [ 0144-8463 ] ; 1994.

RBID : pubmed:8032005

Descripteurs français

KwdFr :
- Concentration en ions d'hydrogène, Diméthylsulfoxyde (pharmacologie), Fusion membranaire, Liposomes, Naphtalènesulfonates, Orthomyxoviridae (physiologie), Polyéthylène glycols (pharmacologie), Sulfones (pharmacologie), Virus parainfluenza humain de type 1 (physiologie).
MESH :
- pharmacologie : Diméthylsulfoxyde, Polyéthylène glycols, Sulfones.
- physiologie : Orthomyxoviridae, Virus parainfluenza humain de type 1.
- Concentration en ions d'hydrogène, Fusion membranaire, Liposomes, Naphtalènesulfonates.

English descriptors

KwdEn :
- Dimethyl Sulfoxide (pharmacology), Hydrogen-Ion Concentration, Liposomes, Membrane Fusion, Naphthalenesulfonates, Orthomyxoviridae (physiology), Parainfluenza Virus 1, Human (physiology), Polyethylene Glycols (pharmacology), Sulfones (pharmacology).
MESH :
- chemical , pharmacology : Dimethyl Sulfoxide, Polyethylene Glycols, Sulfones.
- physiology : Orthomyxoviridae, Parainfluenza Virus 1, Human.
- Hydrogen-Ion Concentration, Liposomes, Membrane Fusion, Naphthalenesulfonates.

Abstract

We have studied the role of hydrophobic interactions in the fusion activity of two lipid enveloped viruses, influenza and Sendai. Using the fluorescent probe ANS (1-aminonaphtalene-8-sulfonate) we have shown that low-pH-dependent influenza virus activation involves a marked increase in the viral envelope hydrophobicity. The effect of dehydrating agents on the fusion activity of both viruses towards model lipid membranes was studied using a fluorescence dequenching assay. Dehydrating agents such as dimethylsulfoxide and dimethylsulfone greatly enhanced the initial rate of the fusion process, the effect of dimethylsulfone doubling that of dimethylsulfoxide. The effect of poly(ethylene glycol) on the fusion process was found to be dependent on the polymer concentration and molecular weight. In general, similar observations were made for both viruses. These results stress the importance of dehydration and hydrophobic interactions in the fusion activity of influenza and Sendai viruses, and show that these factors may be generally involved in membrane fusion events mediated by many other lipid enveloped viruses.

DOI: 10.1007/bf01901634
PubMed: 8032005

Affiliations:

Portugal

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<front><div type="abstract" xml:lang="en">We have studied the role of hydrophobic interactions in the fusion activity of two lipid enveloped viruses, influenza and Sendai. Using the fluorescent probe ANS (1-aminonaphtalene-8-sulfonate) we have shown that low-pH-dependent influenza virus activation involves a marked increase in the viral envelope hydrophobicity. The effect of dehydrating agents on the fusion activity of both viruses towards model lipid membranes was studied using a fluorescence dequenching assay. Dehydrating agents such as dimethylsulfoxide and dimethylsulfone greatly enhanced the initial rate of the fusion process, the effect of dimethylsulfone doubling that of dimethylsulfoxide. The effect of poly(ethylene glycol) on the fusion process was found to be dependent on the polymer concentration and molecular weight. In general, similar observations were made for both viruses. These results stress the importance of dehydration and hydrophobic interactions in the fusion activity of influenza and Sendai viruses, and show that these factors may be generally involved in membrane fusion events mediated by many other lipid enveloped viruses.</div>
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Role of hydrophobic interactions in the fusion activity of influenza and Sendai viruses towards model membranes.

Role of hydrophobic interactions in the fusion activity of influenza and Sendai viruses towards model membranes.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration Stress et Covid
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